Mother Gothel is always touching Rapunzel’s hair
Tangled’s Mother Gothel is evil from the start, which is painfully obvious to the audience. Unfortunately, Rapunzel, sweet but naive, is not as quick to notice the red flags.
The way Mother Gothel always touches her blonde hair when saying “I love you most” is one of the clever ways to show us she doesn’t give a damn about her “daughter.” So she’s basically admitting that she likes Rapunzel’s magical hair more than she likes Rapunzel herself.